Overview

  • Product nameAnti-Piwi antibody
  • Description
    Rabbit polyclonal to Piwi
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide derived from residues 350 - 450 of Drosophila melanogaster Piwi.

    .

  • Positive control
    • This antibody gave a positive signal in Drosophila embryo nuclear extract

Properties

Applications

Our Abpromise guarantee covers the use of ab5207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). Can be blocked with Piwi peptide (ab14803).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevancePiwi mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell. It is not essential for the further differentiation of the committed daughter cell. Acts cell autonomously to promote germline stem cell division. It is expressed somatically in the ovariole terminal filament and epithelial sheath cells. Expressed in the germarium at low levels during oogenesis stages 1-6, at a lower level during stages 7-9, strongly at stage 10 and eventually accumulating in early embryos.
    • Cellular localizationNuclear
    • Database links
      • Alternative names
        • P-element induced wimpy testis antibody
        • Piwi antibody

      Anti-Piwi antibody images

      • Anti-Piwi antibody (ab5207) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 20 µg

        Secondary
        IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/3000 dilution

        Performed under reducing conditions.

        Predicted band size : 97 kDa
        Observed band size : 90 kDa (why is the actual band size different from the predicted?)
        Additional bands at : 53 kDa. We are unsure as to the identity of these extra bands.


      • Predicted band size : 97 kDa

        This image is courtesy of Ahmet Denli

        Drosophila embryo lysate, 8% TRIS SDS-PAGE.
        The arrow indicates Piwi at a predicted MW of 90 kDa.
        Pre-immune sera was used as a control and showed no equivalent band (data not shown).

        Drosophila embryo lysate, 8% TRIS SDS-PAGE. The arrow indicates Piwi at a predicted MW of 90 kDa. Pre-immune sera was used as a control and showed no equivalent band (data not shown). Ahmet Denli

      References for Anti-Piwi antibody (ab5207)

      This product has been referenced in:
      • Patil VS  et al. The Tudor domain protein Tapas, a homolog of the vertebrate Tdrd7, functions in the piRNA pathway to regulate retrotransposons in germline of Drosophila melanogaster. BMC Biol 12:61 (2014). WB ; Drosophila melanogaster . Read more (PubMed: 25287931) »
      • Dufourt J  et al. Spatio-temporal requirements for transposable element piRNA-mediated silencing during Drosophila oogenesis. Nucleic Acids Res N/A:N/A (2013). IHC-P ; Drosophila melanogaster . Read more (PubMed: 24288375) »

      See all 6 Publications for this product

      Product Wall

      Application Immunocytochemistry/ Immunofluorescence
      Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Sample Fruit fly (Drosophila melanogaster) Cell (ovaries)
      Specification ovaries
      Permeabilization Yes - Triton 0,3%
      Fixative Formaldehyde
      Username

      Ms. Nathalie GReD Gueguen

      Verified customer

      Submitted Oct 08 2014

      Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you d...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"