Overview

  • Product name
  • Description
    Rabbit polyclonal to PIWIL1
  • Specificity
    At least three isoforms of PIWIL1 are known to exist; ab105393 will recognize the two longest isoforms. ab105393 is predicted to not cross-react with other PIWI family members.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    18 amino acid synthetic peptide near the N-terminus of Human PIWIL1 (AAK69348).

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab105393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 99 kDa.
ICC Use a concentration of 10 µg/ml.

Target

  • Function
    Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator.
  • Tissue specificity
    Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells.
  • Sequence similarities
    Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Domain
    The PAZ domain specifically recognizes binds the 2'-O-methylated 3'-end of piRNAs.
  • Post-translational
    modifications
    Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
  • Cellular localization
    Cytoplasm. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Also present in chromatoid body.
  • Information by UniProt
  • Database links
  • Alternative names
    • HIWI antibody
    • MIWI antibody
    • Piwi (Drosophila) like 1 antibody
    • PIWI antibody
    • Piwi homolog antibody
    • Piwi like 1 (drosophila) antibody
    • Piwi like 1 antibody
    • Piwi-like protein 1 antibody
    • PIWIL1 antibody
    • PIWL1_HUMAN antibody
    see all

Anti-PIWIL1 antibody images

  • Immunofluorescence of PIWIL1 in rat liver tissue with ab105393 at 20 ug/mL.
  • All lanes : Anti-PIWIL1 antibody (ab105393) at 1 µg/ml

    Lane 1 : HepG2 cell lysate without blocking peptide.
    Lane 2 : HepG2 cell lysate with blocking peptide.

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 99 kDa
  • Immunohistochemical analysis of rat liver tissue, staining PIWIL1 with ab105393 at 10 µg/ml.

References for Anti-PIWIL1 antibody (ab105393)

This product has been referenced in:
  • Chen Z  et al. Stem cell protein Piwil1 endowed endometrial cancer cells with stem-like properties via inducing epithelial-mesenchymal transition. BMC Cancer 15:811 (2015). IHC . Read more (PubMed: 26506848) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization
Yes - Wash Buffer from Dako with Tween
Specification
Breast
Fixative
Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

Submitted Mar 08 2016

Abreviews
Application
RIP
Sample
Cow Cell lysate - whole cell (Testis)
Negative control
2 µg non-specific rabbit IgG (Milipore, #12-370)
Immuno-precipitation step
Protein A
Specification
Testis
Detection step
Other
Type
RIP without cross-linking
Duration of cross-linking step: 0 second(s)
Positive control
Testis with 3 µg Ab105393 Anti-PIWIL1
Username

Dr. Stewart Russell

Verified customer

Submitted Oct 16 2015

Application
Western blot
Sample
Cow Tissue lysate - whole (Testis)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Testis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Stewart Russell

Verified customer

Submitted Jul 14 2015

Thank you for contacting us.

The sequence homology between human and Platypus species seems quite OK for antibodies ab12337 and ab85084 cross reactivity. These antibodies may be tried for further tests. We can provide abrreview discount for th...

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