Overview

  • Product name
  • Description
    Rabbit polyclonal to PIWIL2
  • Tested applications
    Suitable for: IHC-Fr, WB, Flow Cyt, ICC/IF, IP, Electron Microscopymore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse MILI/ HILI.

    (Peptide available as ab27821.)

  • Positive control
    • Mouse Testis Whole Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab36764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 20534472
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).Can be blocked with Mouse PIWIL2 peptide (ab27821).
Flow Cyt Use at an assay dependent concentration. PubMed: 20460113ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 20460113
IP Use at an assay dependent concentration. PubMed: 20460113
Electron Microscopy Use at an assay dependent concentration. PubMed: 20011505

Target

  • Function
    Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors.
  • Tissue specificity
    Expressed in adult testis and in most tumors.
  • Sequence similarities
    Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Post-translational
    modifications
    Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule.
  • Cellular localization
    Cytoplasm. Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 80 antibody
    • CT80 antibody
    • Hiwi like antibody
    • MILI antibody
    • Miwi like antibody
    • Piwi like 2 antibody
    • Piwi-like protein 2 antibody
    • Piwil2 antibody
    • PIWL2_HUMAN antibody
    see all

Anti-PIWIL2 antibody images

  • Anti-PIWIL2 antibody (ab36764) at 1 µg/ml + Mouse Testis Whole Tissue Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • ab36764 staining PIWIL2 in murine testis tissue by Immunohistochemistry (Frozen sections). Chromatin was stained with DAPI.

References for Anti-PIWIL2 antibody (ab36764)

This product has been referenced in:
  • Feng D  et al. Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis. Oncotarget 7:64575-64588 (2016). IP ; Human . Read more (PubMed: 27602489) »
  • Zhou J  et al. STK31/TDRD8, a germ cell-specific factor, is dispensable for reproduction in mice. PLoS One 9:e89471 (2014). IF ; Mouse . Read more (PubMed: 24586802) »

See all 9 Publications for this product

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Thank you for contacting us.

The Western Blot image was obtained using 1 µg/ml of ab36764.

The IHC-Fr image comes from a publication : http://www.ncbi.nlm.nih.gov/pubmed/20534472?dopt=Abstract. Unfortunately there is no infor...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Testis)
Loading amount
30 µg
Specification
Testis
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Feb 04 2008

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