Anti-PIWIL2 antibody (ab85084)
Key features and details
- Rabbit polyclonal to PIWIL2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PIWIL2 antibody
See all PIWIL2 primary antibodies -
Description
Rabbit polyclonal to PIWIL2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee -
Immunogen
Synthetic peptide:
VITRYNNRTYRIDDVD
, corresponding to internal sequence amino acids 418-433 of Human PIWIL2 -
Positive control
- Testis tissue. Human fetal testis lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 0.184% Tris glycine -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/500. Predicted molecular weight: 110 kDa.
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IHC-P |
1/100 - 1/400.
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Notes |
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WB
1/200 - 1/500. Predicted molecular weight: 110 kDa. |
IHC-P
1/100 - 1/400. |
Target
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Function
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. -
Tissue specificity
Expressed in adult testis and in most tumors. -
Sequence similarities
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Post-translational
modificationsArginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule. -
Cellular localization
Cytoplasm. Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. - Information by UniProt
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Database links
- Entrez Gene: 472711 Chimpanzee
- Entrez Gene: 486125 Dog
- Entrez Gene: 55124 Human
- Entrez Gene: 57746 Mouse
- Entrez Gene: 306011 Rat
- Omim: 610312 Human
- SwissProt: Q8TC59 Human
- SwissProt: Q8CDG1 Mouse
see all -
Alternative names
- Cancer/testis antigen 80 antibody
- CT80 antibody
- Hiwi like antibody
see all
Images
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Immunohistochemistry analysis of PIWIL2 in formalin-fixed paraffin-embedded human fetal testis tissue sections using ab85084 at 1/200 dilution.
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Anti-PIWIL2 antibody (ab85084) at 1/200 dilution + Human fetal testis lysate
Predicted band size: 110 kDa
Observed band size: 110 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab85084 has been referenced in 6 publications.
- Feng D et al. CBP-mediated Wnt3a/ß-catenin signaling promotes cervical oncogenesis initiated by Piwil2. Neoplasia 23:1-11 (2021). PubMed: 33190089
- Wang S et al. Interferon-inducible protein, IFIX, has tumor-suppressive effects in oral squamous cell carcinoma. Sci Rep 11:19593 (2021). PubMed: 34599264
- Ma C et al. piRNA-63076 contributes to pulmonary arterial smooth muscle cell proliferation through acyl-CoA dehydrogenase. J Cell Mol Med 24:5260-5273 (2020). PubMed: 32227582
- Wang S et al. Expression of PIWIL2 in oral cancer and leukoplakia: Prognostic implications and insights from tumors. Cancer Biomark 26:11-20 (2019). PubMed: 31322538
- Feng D et al. Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis. Oncotarget 7:64575-64588 (2016). WB, IHC ; Human . PubMed: 27602489
- Berdasco M et al. DNA Methylation Plasticity of Human Adipose-Derived Stem Cells in Lineage Commitment. Am J Pathol : (2012). WB . PubMed: 23031258