Anti-PIWIL3 antibody (ab77088)
Key features and details
- Mouse polyclonal to PIWIL3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PIWIL3 antibody
See all PIWIL3 primary antibodies -
Description
Mouse polyclonal to PIWIL3 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length human PIWIL3 (NP_001008496, aa 1-882).
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Positive control
- PIWIL3 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
Target
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Function
May play a role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. -
Tissue specificity
Expressed in testis. -
Sequence similarities
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Cellular localization
Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. - Information by UniProt
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Database links
- Entrez Gene: 440822 Human
- Omim: 610314 Human
- SwissProt: Q7Z3Z3 Human
- Unigene: 448343 Human
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Alternative names
- HIWI3 antibody
- Piwi like 3 antibody
- Piwi-like protein 3 antibody
see all
Images
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All lanes : Anti-PIWIL3 antibody (ab77088) at 5 µg/ml
Lane 1 : PIWIL3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 40 kDa, 60 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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Datasheet download
References (3)
ab77088 has been referenced in 3 publications.
- Abdelhamid RF et al. piRNA/PIWI Protein Complex as a Potential Biomarker in Sporadic Amyotrophic Lateral Sclerosis. Mol Neurobiol 59:1693-1705 (2022). PubMed: 35015250
- Liu J et al. piRNA-36741 regulates BMP2-mediated osteoblast differentiation via METTL3 controlled m6A modification. Aging (Albany NY) 13:23361-23375 (2021). PubMed: 34645714
- Hashim A et al. RNA sequencing identifies specific PIWI-interacting small non-coding RNA expression patterns in breast cancer. Oncotarget 5:9901-10 (2014). PubMed: 25313140