Overview

  • Product nameAnti-PIWIL3 antibody
    See all PIWIL3 primary antibodies
  • Description
    Mouse polyclonal to PIWIL3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human PIWIL3 (NP_001008496, aa 1-882).

  • Positive control
    • PIWIL3 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).

Target

  • FunctionMay play a role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation.
  • Tissue specificityExpressed in testis.
  • Sequence similaritiesBelongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Cellular localizationCytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • HIWI3 antibody
    • Piwi like 3 antibody
    • Piwi-like protein 3 antibody
    • piwi-like RNA-mediated gene silencing 3 antibody
    • PIWIL3 antibody
    • PIWL3_HUMAN antibody
    see all

Anti-PIWIL3 antibody images

  • All lanes : Anti-PIWIL3 antibody (ab77088) at 5 µg/ml

    Lane 1 : PIWIL3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
    Additional bands at : 40 kDa,60 kDa,70 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PIWIL3 antibody (ab77088)

ab77088 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and for your patience.

We have sourced andadded a new transfected 293T cell lysate to our catalog for you. I can recommend to review the online datasheet for further information:

ab131153 PIWIL3 293T Tra...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"