Overview

  • Product nameAnti-PIWIL3 antibody
    See all PIWIL3 primary antibodies
  • Description
    Rabbit polyclonal to PIWIL3
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PIWIL3 (internal sequence).
    Database link: NM_001008496

  • Positive control
    • Human testis tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab93709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
  • Application notesIHC-P: 1/100 - 1/500.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation.
    • Tissue specificityExpressed in testis.
    • Sequence similaritiesBelongs to the argonaute family. Piwi subfamily.
      Contains 1 PAZ domain.
      Contains 1 Piwi domain.
    • Cellular localizationCytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • HIWI3 antibody
      • Piwi like 3 antibody
      • Piwi-like protein 3 antibody
      • piwi-like RNA-mediated gene silencing 3 antibody
      • PIWIL3 antibody
      • PIWL3_HUMAN antibody
      see all

    Anti-PIWIL3 antibody images

    • ICC/IF image of ab93709 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93709 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • ab93709, at 1/100 dilution, staining PIWIL3 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry.

    References for Anti-PIWIL3 antibody (ab93709)

    ab93709 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    I can confirm that the immunogen for ab93709 is a peptide located between amino acids 500 and 600of human PIWIL3(SwissProtID Q7Z3Z3):

    PLLNAMPLHSWLILYSRSSHREAMSLKGHLQSVTAPMGITMKPAEMIEVDGDANSYIDTLRKYTRPTLQ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"