Overview

  • Product nameAnti-PIWIL4 antibody
    See all PIWIL4 primary antibodies
  • Description
    Rabbit polyclonal to PIWIL4
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 639-839 of Human PIWIL4 (AAH31060).

  • Positive control
    • MCF7 and IMR32 cells and whole cell lysates; 59T xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab111714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 97 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0)
ICC/IF 1/100 - 1/500.

Target

  • FunctionPlays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.
  • Tissue specificityExpressed in testis. According to PubMed:17544373, it is ubiquitously expressed.
  • Sequence similaritiesBelongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Post-translational
    modifications
    Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
  • Cellular localizationNucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686P01248 antibody
    • FLJ36156 antibody
    • HILI 2 antibody
    • HILI2 antibody
    • HIWI 2 antibody
    • HIWI2 antibody
    • Miwi 2 protein antibody
    • Miwi2 antibody
    • PIWI antibody
    • Piwi like 2 antibody
    • Piwi like 4 (Drosophila) antibody
    • Piwi like 4 antibody
    • Piwi like protein 4 antibody
    • PIWI like protein antibody
    • Piwi like RNA mediated gene silencing 4 antibody
    • Piwi-like protein 4 antibody
    • PIWIL 4 antibody
    • Piwil4 antibody
    • PIWL4_HUMAN antibody
    see all

Anti-PIWIL4 antibody images

  • Anti-PIWIL4 antibody (ab111714) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size : 97 kDa
  • ab111714 at 1/200 dilution staining PIWIL4 in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
  • ab111714 at 1/500 dilution staining PIWIL4 in Formalin-fixed, paraffin-embedded 59T xenograft tissue by Immunohistochemistry.

References for Anti-PIWIL4 antibody (ab111714)

ab111714 has not yet been referenced specifically in any publications.

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