Overview

  • Product name
  • Description
    Rabbit polyclonal to PIWIL4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human PIWIL4. (NP_689644.2)
    Database link: Q7Z3Z4

  • Positive control
    • Human Testis, Spermatogonia tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab189356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 97 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.
  • Tissue specificity
    Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed.
  • Sequence similarities
    Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Post-translational
    modifications
    Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
  • Cellular localization
    Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • HILI2 antibody
    • HIWI2 antibody
    • MISTI antibody
    • MIWI2 antibody
    • MIWI2, mouse, homolog of antibody
    • piwi-like 4 antibody
    • Piwi-like protein 4 antibody
    • Piwi-like RNA-mediated gene silencing 4 antibody
    • Piwil4 antibody
    • PIWL4_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Testis, Spermatogonia tissue labeling PIWIL4 with ab189356 at 10 µg/ml.

References

ab189356 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189356.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up