PKA 2 beta (regulatory subunit) antibody (ab28256)

Product Name 

PKA 2 beta (regulatory subunit) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PKA 2 beta (regulatory subunit)

Immunogen 

Synthetic peptide (Rat) from PKA 2 beta (regulatory subunit).(Peptide available as ab41298.)

Reacts with 
(species key)

Hu, Ms, Rat, Rb

Specificity 

ab28256 recognizes Protein Kinase A Regulatory Subunit IIb. It does not recognize the other PKA regulatory subunits (PKARIa, PKARIIa, PKARIb).

We have a range of domain specific antibodies for this target. For a full list please see all PKA 2 beta (regulatory subunit) antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000, when using colorometric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Predicted molecular weight: 46 kDa. But note that when used against the reduced protein, ab28256 identifies bands at 55 kDa and 53 kDa, as well as activation/breakdown products. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA

Relevance 

PKA 2 beta (regulatory subunit or PRKAR2B) is one of the regulatory subunits of cAMP-dependent Protein Kinase (PKA), which is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits (R1 alpha, R1 beta, R2 alpha, and R2 beta). Upon adenylate cyclase activation, AMP binds to the regulatory subunits, and releases the catalytic subunit, which becomes active upon its dissociation from the regulatory subunit.
This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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