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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-PKA 2 beta (regulatory subunit) antibody(ab28256)
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Product Name
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PKA 2 beta (regulatory subunit) antibody
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See all PKA 2 beta (regulatory subunit) antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PKA 2 beta (regulatory subunit)
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Immunogen
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Synthetic peptide (Rat) from PKA 2 beta (regulatory subunit).(Peptide available as ab41298.)
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Reacts with
(species key)
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Hu, Ms, Rat, Rb
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Specificity
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ab28256 recognizes Protein Kinase A Regulatory Subunit IIb. It does not recognize the other PKA regulatory subunits (PKARIa, PKARIIa, PKARIb).
We have a range of domain specific antibodies for this target. For a full list please see all PKA 2 beta (regulatory subunit) antibodies
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000, when using colorometric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Predicted molecular weight: 46 kDa. But note that when used against the reduced protein, ab28256 identifies bands at 55 kDa and 53 kDa, as well as activation/breakdown products. Dilution optimised using Chromogenic detection.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
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Relevance
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PKA 2 beta (regulatory subunit or PRKAR2B) is one of the regulatory subunits of cAMP-dependent Protein Kinase (PKA), which is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits (R1 alpha, R1 beta, R2 alpha, and R2 beta). Upon adenylate cyclase activation, AMP binds to the regulatory subunits, and releases the catalytic subunit, which becomes active upon its dissociation from the regulatory subunit.
This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody has been peptide-affinity purified.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PKA 2 beta (regulatory subunit) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PKA 2 beta (regulatory subunit) antibody images, references, products related to ab28256 and other tools.
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PKA 2 beta (regulatory subunit) antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-PKA 2 beta (regulatory subunit) antibody(ab28256)
Ab28256 staining human normal breast tissue. Staining is localized to cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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Search PubMed (MEDLINE) for references to PKA 2 beta (regulatory subunit)
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PKA 2 beta (regulatory subunit) antibody - more information
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