Anti-PKA 2 beta (regulatory subunit) antibody [EP2648] (ab75996)

Overview

  • Product nameAnti-PKA 2 beta (regulatory subunit) antibody [EP2648]
    See all PKA 2 beta (regulatory subunit) primary antibodies
  • Description
    Rabbit monoclonal [EP2648] to PKA 2 beta (regulatory subunit)
  • Tested applicationsSuitable for: IHC-Fr, WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N terminus of human PKA 2 beta (regulatory subunit)

  • Positive control
    • Fetal brain, human testis and NIH/3T3 cell lysates. Human brain tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP2648
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab75996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22649251
WB 1/5000 - 1/20000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionRegulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
    • Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
    • Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
      Contains 2 cyclic nucleotide-binding domains.
    • Post-translational
      modifications
      Phosphorylated by the activated catalytic chain.
    • Cellular localizationCytoplasm. Cell membrane. Co-localizes with PJA2 in the cytoplasm and at the cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI451071 antibody
      • AW061005 antibody
      • cAMP dependent protein kinase type II beta regulatory chain antibody
      • cAMP dependent protein kinase type II beta regulatory subunit antibody
      • cAMP-dependent protein kinase type II-beta regulatory subunit antibody
      • H RG363E19.2 antibody
      • KAP3_HUMAN antibody
      • MGC116401 antibody
      • Pkarb2 antibody
      • PRKAR 2 antibody
      • PRKAR2 antibody
      • PRKAR2B antibody
      • Protein kinase cAMP dependent regulatory type II beta antibody
      • RATDNA antibody
      • RII beta antibody
      • RII(beta) antibody
      • RIIbeta antibody
      • WUGSC:H RG363E19.2 antibody
      see all

    Anti-PKA 2 beta (regulatory subunit) antibody [EP2648] images

    • All lanes : Anti-PKA 2 beta (regulatory subunit) antibody [EP2648] (ab75996) at 1/20000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Human testis lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
    • Overlay histogram showing SHSY-5Y cells stained with ab75996 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75996, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • ab75996, at 1/100 dilution, staining PKA 2 beta (regulatory subunit) in paraffin-embedded human brain tissue by Immunohistochemistry.

    References for Anti-PKA 2 beta (regulatory subunit) antibody [EP2648] (ab75996)

    This product has been referenced in:
    • Corredor RG  et al. Soluble adenylyl cyclase activity is necessary for retinal ganglion cell survival and axon growth. J Neurosci 32:7734-44 (2012). IHC-Fr ; Rat . Read more (PubMed: 22649251) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"