Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993)


  • Product nameAnti-PKA 2 beta (regulatory subunit) antibody [EP2649Y]
    See all PKA 2 beta (regulatory subunit) primary antibodies
  • Description
    Rabbit monoclonal [EP2649Y] to PKA 2 beta (regulatory subunit)
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PKA 2 beta (regulatory subunit) (C terminal).

  • Positive control
    • WB: Human brain lysate IHC-P: Human testis tissue
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP2649Y
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab75993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: 1/80.
    ICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP conjugated or polymerized HRP secondary antibodies recommended. Stronger signals have been found using the polymerized HRP secondary.
    IP: 1/60.
    WB: 1/25,000 - 1/50,000. Predicted molecular weight: 46 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRegulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
    • Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
    • Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
      Contains 2 cyclic nucleotide-binding domains.
    • Post-translational
      Phosphorylated by the activated catalytic chain.
    • Cellular localizationCytoplasm. Cell membrane. Co-localizes with PJA2 in the cytoplasm and at the cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI451071 antibody
      • AW061005 antibody
      • cAMP dependent protein kinase type II beta regulatory chain antibody
      • cAMP dependent protein kinase type II beta regulatory subunit antibody
      • cAMP-dependent protein kinase type II-beta regulatory subunit antibody
      • H RG363E19.2 antibody
      • KAP3_HUMAN antibody
      • MGC116401 antibody
      • Pkarb2 antibody
      • PRKAR 2 antibody
      • PRKAR2 antibody
      • PRKAR2B antibody
      • Protein kinase cAMP dependent regulatory type II beta antibody
      • RATDNA antibody
      • RII beta antibody
      • RII(beta) antibody
      • RIIbeta antibody
      • WUGSC:H RG363E19.2 antibody
      see all

    Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] images

    • Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993) at 1/50000 dilution + Human brain lysate at 10 µg

      goat anti rabbit HRP at 1/2000 dilution

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
    • ab75993, at a 1/100 dilution, staining PKA 2 beta (regulatory subunit) in formalin fixed, paraffin embedded human testis tissue by Immunohistochemistry.
    • Overlay histogram showing SH-SY5Y cells stained with ab75993 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75993, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References for Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993)

    This product has been referenced in:
    • Nagalla S  et al. Platelet microRNA-mRNA coexpression profiles correlate with platelet reactivity. Blood 117:5189-97 (2011). WB ; Human . Read more (PubMed: 21415270) »

    See 1 Publication for this product

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