Anti-PKA 2 beta (regulatory subunit) (phospho S114) antibody (ab5829)

Overview

  • Product nameAnti-PKA 2 beta (regulatory subunit) (phospho S114) antibody
    See all PKA 2 beta (regulatory subunit) primary antibodies
  • Description
    Rabbit polyclonal to PKA 2 beta (regulatory subunit) (phospho S114)
  • SpecificityPKA regulatory 2 alpha subunit (75% homologous) has not been tested.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow
  • Immunogen

    Synthetic phosphopeptide derived from a region of human PKA regulatory 2 beta subunit that contains serine 114.

  • Positive control
    • 3T3-L1 adipocytes or Y1 mouse adrenal cortical cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKA. The final product is generated by affinity chromatography using a PKA-derived peptide that is phosphorylated at serine 114.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 50 kDa.

Target

  • FunctionRegulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
  • Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
  • Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.
  • Post-translational
    modifications
    Phosphorylated by the activated catalytic chain.
  • Cellular localizationCytoplasm. Cell membrane. Co-localizes with PJA2 in the cytoplasm and at the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI451071 antibody
    • AW061005 antibody
    • cAMP dependent protein kinase type II beta regulatory chain antibody
    • cAMP dependent protein kinase type II beta regulatory subunit antibody
    • cAMP-dependent protein kinase type II-beta regulatory subunit antibody
    • H RG363E19.2 antibody
    • KAP3_HUMAN antibody
    • MGC116401 antibody
    • Pkarb2 antibody
    • PRKAR 2 antibody
    • PRKAR2 antibody
    • PRKAR2B antibody
    • Protein kinase cAMP dependent regulatory type II beta antibody
    • RATDNA antibody
    • RII beta antibody
    • RII(beta) antibody
    • RIIbeta antibody
    • WUGSC:H RG363E19.2 antibody
    see all

Anti-PKA 2 beta (regulatory subunit) (phospho S114) antibody images

  • Peptide Competition and Phosphatase Treatment: Lysates prepared from 3T3-L1 cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for two hours at room temperature, and incubated with ab5829 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKA reg 2 beta [pS114] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

References for Anti-PKA 2 beta (regulatory subunit) (phospho S114) antibody (ab5829)

ab5829 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"