Anti-PKA alpha + beta (catalytic subunits) antibody - N-terminal (ab71764)

Overview

  • Product nameAnti-PKA alpha + beta (catalytic subunits) antibody - N-terminal
    See all PKA alpha + beta (catalytic subunits) primary antibodies
  • Description
    Rabbit polyclonal to PKA alpha + beta (catalytic subunits) - N-terminal
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from the N-terminal region, between amino acids 10-40, of human PKA alpha + beta (catalytic subunits), conjugated to KLH

  • Positive control
    • Jurkat cell and mouse heart tissue lysates and human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 35 kDa (predicted molecular weight: 41 kDa).

Target

  • RelevancePRKACA and PRKACB are members of the Ser/Thr protein kinase family and are a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
  • Cellular localizationCytoplasm. Nucleus. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm
  • Database links
  • Alternative names
    • cAMP dependent protein kinase beta catalytic subunit antibody
    • cAMP dependent protein kinase alpha catalytic subunit antibody
    • cAMP dependent protein kinase catalytic subunit alpha antibody
    • cAMP dependent protein kinase catalytic subunit beta antibody
    • PKA C alpha antibody
    • PKA C beta antibody
    • PKACA antibody
    • PKACB antibody
    • PRKACA antibody
    • PRKACB antibody
    • Protein kinase cAMP dependent catalytic alpha antibody
    • Protein kinase cAMP dependent catalytic beta antibody
    see all

Anti-PKA alpha + beta (catalytic subunits) antibody - N-terminal images

  • All lanes : Anti-PKA alpha + beta (catalytic subunits) antibody - N-terminal (ab71764) at 1/100 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : mouse heart tissue lysate

    Lysates/proteins at 12.5 µg per lane.


    Predicted band size : 41 kDa
    Observed band size : 34,35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
  • ab71764 at 1/50 dilution, staining PKA alpha + beta (catalytic subunits) in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

References for Anti-PKA alpha + beta (catalytic subunits) antibody - N-terminal (ab71764)

ab71764 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"