Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218)

Overview

  • Product nameAnti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody
    See all PKA alpha + beta (catalytic subunits) primary antibodies
  • Description
    Rabbit polyclonal to PKA alpha + beta (catalytic subunits) (phospho T197)
  • Specificityab59218 detects endogenous levels of PKA alpha + beta (catalytic subunit) only when phosphorylated at threonine 197.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide derived from human PKA alpha + beta (catalytic subunit)around the phosphorylation site of threonine 197 (T-W-TP-L-C).

  • Positive control
    • Human colon carcinoma tissue and mouse brain cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab59218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

  • RelevancePRKACA and PRKACB are members of the Ser/Thr protein kinase family and are a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
  • Cellular localizationCytoplasm. Nucleus. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm
  • Database links
  • Alternative names
    • cAMP dependent protein kinase beta catalytic subunit antibody
    • cAMP dependent protein kinase alpha catalytic subunit antibody
    • cAMP dependent protein kinase catalytic subunit alpha antibody
    • cAMP dependent protein kinase catalytic subunit beta antibody
    • PKA C alpha antibody
    • PKA C beta antibody
    • PKACA antibody
    • PKACB antibody
    • PRKACA antibody
    • PRKACB antibody
    • Protein kinase cAMP dependent catalytic alpha antibody
    • Protein kinase cAMP dependent catalytic beta antibody
    see all

Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody images

  • ab59218 at 1/50 dilution staining PKA alpha + beta (catalytic subunit) in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence (left) or presence (right) of the immunising peptide.
  • All lanes : Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218) at 1/500 dilution

    Lane 1 : mouse brain cell extract
    Lane 2 : mouse brain cell extract with immunising peptide


    Predicted band size : 41 kDa
    Observed band size : 41 kDa

References for Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218)

ab59218 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"