The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 41 kDa). Other bands are detected when using extracts from CoLo205 cells.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.
Tissue specificityIsoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.
Post-translational modificationsAsn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.
Cellular localizationCytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.
cAMP-dependent protein kinase catalytic beta subunit isoform 4ab antibody
cAMP-dependent protein kinase catalytic subunit beta antibody
PKA C beta antibody
PKA C-beta antibody
protein kinase A catalytic subunit beta antibody
Protein kinase cAMP dependent catalytic beta antibody
Anti-PKA beta (catalytic subunit) antibody images
Western blot - Anti-PKA beta (catalytic subunit) antibody (ab64801)
All lanes : Anti-PKA beta (catalytic subunit) antibody (ab64801) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells at 5-30ug with no immunizing peptide Lane 2 : Extracts from CoLo205 cells at 5-30ug with no immunizing peptide Lane 3 : CoLo205 cells at 5-30ug with immunizing peptide at 5-10ug