Anti-PKA beta (catalytic subunit) (phospho S338) antibody (ab5816)


  • Product name
    Anti-PKA beta (catalytic subunit) (phospho S338) antibody
    See all PKA beta (catalytic subunit) primary antibodies
  • Description
    Rabbit polyclonal to PKA beta (catalytic subunit) (phospho S338)
  • Specificity
    Peptide competition data indicate that this antibody cross-reacts with the PKA nu subunit (64% homologous) and partially with the alpha subunit (82% homologous).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic phosphopeptide derived from a region of human PKA catalytic beta subunit that contains serine 338.

  • Positive control
    • 3T3-L1 adipocytes.
  • General notes

    c-AMP-dependent Protein Kinase (PKA) is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits that dissociate upon activation by cAMP. The catalytic subunit of PKA contains the activation loop and mediates DNA binding and substrate recognition. The catalytic subunit is assembled and expressed as an active form and is phosphorylated on threonine 197 by PDK 1 in the activation loop and serine 338 in the carboxyl terminus. Phosphorylation of serine 338 plays a key role in stabilizing PKA and activating its substrates, and hence mediating its biological functions.



Our Abpromise guarantee covers the use of ab5816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 42 kDa.


  • Function
    Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.
  • Tissue specificity
    Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.
  • Cellular localization
    Cytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP-dependent protein kinase catalytic beta subunit isoform 4ab antibody
    • cAMP-dependent protein kinase catalytic subunit beta antibody
    • KAPCB_HUMAN antibody
    • PKA C beta antibody
    • PKA C-beta antibody
    • PKACB antibody
    • Prkacb antibody
    • protein kinase A catalytic subunit beta antibody
    • Protein kinase cAMP dependent catalytic beta antibody
    see all


  • Peptide Competition: Lysates prepared from 3T3-L1 cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either treated with lambda phosphatase (1) or left untreated (2-5), blocked with a 5% BSA-TBSTbuffer for two hours at room temperature, and incubated with ab5816 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP-conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKA cat beta [pS338] blocks the antibody signal, thereby verifying the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.


ab5816 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry. Stimulation is NOT required for the detection of PKA beta (catalytic subunit) (phospho S338) by ab5816.

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you...

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