Overview

  • Product nameAnti-PKA R2 antibody [5F1]
    See all PKA R2 primary antibodies
  • Description
    Mouse monoclonal [5F1] to PKA R2
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length Human PKA R2 produced in HEK293T cells (NP_004148).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY PKA R2; HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, and MCF7 cell extracts; Human prostate tissue; COS7 cells transiently transfected with pCMV6-ENTRY PKA R2; HEK293T cells transfected with a PKA R2 overexpress plasmid
  • General notesDilute in PBS (pH7.3) before use. Avoid repeated freeze-thaws.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 45 kDa.
IHC-P 1/50.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionRegulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
  • Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
  • Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.
  • Post-translational
    modifications
    Phosphorylated by the activated catalytic chain.
  • Cellular localizationCytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP dependent protein kinase regulatory subunit alpha 2 antibody
    • cAMP dependent protein kinase regulatory subunit RII alpha antibody
    • cAMP dependent protein kinase type II alpha regulatory chain antibody
    • cAMP dependent protein kinase type II alpha regulatory subunit antibody
    • cAMP-dependent protein kinase type II-alpha regulatory subunit antibody
    • KAP2 antibody
    • KAP2_HUMAN antibody
    • MGC3606 antibody
    • PKR 2 antibody
    • PKR2 antibody
    • PRKA R2 antibody
    • PRKAR 2 antibody
    • PRKAR2 antibody
    • PRKAR2A antibody
    • Protein kinase A RII alpha subunit antibody
    • Protein kinase cAMP dependent regulatory type II alpha antibody
    see all

Anti-PKA R2 antibody [5F1] images

  • All lanes : Anti-PKA R2 antibody [5F1] (ab124400) at 1/1000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PKA R2

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 45 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205473)
  • All lanes : Anti-PKA R2 antibody [5F1] (ab124400) at 1/1000 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 45 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC205471)
  • ab124400, at 1/50 dilution, staining PKA R2 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
  • ab124400, at 1/100 dilution, staining PKA R2 in COS7 cells transiently transfected with pCMV6-ENTRY PKA R2 by Immunofluorescence.
  • HEK293T cells transfected with either a PKA R2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab124400 at 1/100 dilution, and then analyzed by flow cytometry.

References for Anti-PKA R2 antibody [5F1] (ab124400)

This product has been referenced in:
  • Hedrick ED  et al. Differential PKA activation and AKAP association determines cell fate in cancer cells. J Mol Signal 8:10 (2013). Human . Read more (PubMed: 24083380) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"