Overview

  • Product nameAnti-PKA R2 antibody [Y116]
    See all PKA R2 primary antibodies
  • Description
    Rabbit monoclonal [Y116] to PKA R2
  • SpecificityThe antibody recognises PKA RII. It does not cross-react with other cAMP-dependent kinase.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C-terminus of human cAMP-dependent protein kinase type II-alpha regulatory subunit.

  • Positive control
    • K562 cell lysate, HeLa cells and normal breast tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionRegulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
    • Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
    • Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
      Contains 2 cyclic nucleotide-binding domains.
    • Post-translational
      modifications
      Phosphorylated by the activated catalytic chain.
    • Cellular localizationCytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • cAMP dependent protein kinase regulatory subunit alpha 2 antibody
      • cAMP dependent protein kinase regulatory subunit RII alpha antibody
      • cAMP dependent protein kinase type II alpha regulatory chain antibody
      • cAMP dependent protein kinase type II alpha regulatory subunit antibody
      • cAMP-dependent protein kinase type II-alpha regulatory subunit antibody
      • KAP2 antibody
      • KAP2_HUMAN antibody
      • MGC3606 antibody
      • PKR 2 antibody
      • PKR2 antibody
      • PRKA R2 antibody
      • PRKAR 2 antibody
      • PRKAR2 antibody
      • PRKAR2A antibody
      • Protein kinase A RII alpha subunit antibody
      • Protein kinase cAMP dependent regulatory type II alpha antibody
      see all

    Anti-PKA R2 antibody [Y116] images

    • Anti-PKA R2 antibody [Y116] (ab32514) at 1/50000 dilution + K562 cell lysate

      Predicted band size : 45 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human normal breast tissue using ab32514 at 1/100 dilution.
    • Immunofluorescent staining of HeLa cells using ab32514 at 1/100 dilution.

    References for Anti-PKA R2 antibody [Y116] (ab32514)

    This product has been referenced in:
    • Hillman P  et al. Dissociation between AKAP3 and PKARII promotes AKAP3 degradation in sperm capacitation. PLoS One 8:e68873 (2013). WB ; Cow . Read more (PubMed: 23894359) »
    • Gu L  et al. Protein kinase A activation confers resistance to trastuzumab in human breast cancer cell lines. Clin Cancer Res 15:7196-206 (2009). IHC-P ; Human . Read more (PubMed: 19920112) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"