Anti-PKA regulatory subunit I beta antibody (ab167325)

Overview

  • Product name
    Anti-PKA regulatory subunit I beta antibody
    See all PKA regulatory subunit I beta primary antibodies
  • Description
    Mouse polyclonal to PKA regulatory subunit I beta
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-381 of Human PKA regulatory subunit I beta (NP_002726.1).

  • Positive control
    • PKA regulatory subunit I beta transfected 293T cell lysate; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab167325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Tissue specificity
    Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
  • Sequence similarities
    Belongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.
  • Post-translational
    modifications
    The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP dependent protein kinase type I beta regulatory subunit antibody
    • cAMP-dependent protein kinase type I-beta regulatory subunit antibody
    • KAP1_HUMAN antibody
    • PKARI beta antibody
    • PRKAR 1 antibody
    • PRKAR 1B antibody
    • PRKAR1 antibody
    • Prkar1b antibody
    • PRKAR1B protein antibody
    • Protein kinase cAMP dependent regulatory type I beta antibody
    • RIbeta antibody
    see all

Images

  • All lanes : Anti-PKA regulatory subunit I beta antibody (ab167325) at 1 µg/ml

    Lane 1 : PKA regulatory subunit I beta transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG HRP at 1/2500 dilution

    Predicted band size: 43 kDa

  • Immunofluorescent analysis of PKA regulatory subunit I beta expression in HeLa cells using ab167325 at 10 µg/ml dilution.

References

ab167325 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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