PKA regulatory subunit I beta antibody (ab28544)

Product Name 

PKA regulatory subunit I beta antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PKA regulatory subunit I beta

Immunogen 

Synthetic peptide, corresponding to amino acids 60-90 of Rat PKA regulatory subunit I beta(Peptide available as ab41296.)

Reacts with 
(species key)

Hu, Ms, Rat, Rb

Specificity 

ab28544 binds to PKARIb, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIIa, PKARIIb).

We have a range of domain specific antibodies for this target. For a full list please see all PKA regulatory subunit I beta antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. Predicted molecular weight: 43 kDa.
This antibody identifies bands at 55 kD and 53 kD when used against the reduced protein as well as activation/breakdown products. The antibody can recognize native or reduced PKARIb. Dilution optimised using Chromogenic detection.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKA
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other

Relevance 

Protein Kinase A is a critical kinase involved in numerous biological functions. The regulatory subunits of PKA allow a range of modifying agents to influence PKA activity and localization, and are found in different levels in a tissue specific manner. The type 1(RI) and type 2 (RII) subunit of cyclic AMP dependent protein kinase A (PKA) is a polyfunctional protein that is present in most tissues and cells.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: Sodium Azide
Constituents: 50% Sucrose, PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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