Overview

  • Product nameAnti-PKA regulatory subunit I beta antibody
    See all PKA regulatory subunit I beta primary antibodies
  • Description
    Rabbit polyclonal to PKA regulatory subunit I beta
  • SpecificityThis antibody is specific for PKA regulatory subunit I beta.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the sequence of human PKA regulatory subunit I beta within aa 1-100.

  • Positive control
    • Human breast carcinoma tissue and mouse liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab38225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Tissue specificityFour types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
  • Sequence similaritiesBelongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.
  • Post-translational
    modifications
    The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • cAMP dependent protein kinase type I beta regulatory subunit antibody
    • cAMP-dependent protein kinase type I-beta regulatory subunit antibody
    • KAP1_HUMAN antibody
    • PKARI beta antibody
    • PRKAR 1 antibody
    • PRKAR 1B antibody
    • PRKAR1 antibody
    • Prkar1b antibody
    • PRKAR1B protein antibody
    • Protein kinase cAMP dependent regulatory type I beta antibody
    • RIbeta antibody
    see all

Anti-PKA regulatory subunit I beta antibody images

  • Anti-PKA regulatory subunit I beta antibody (ab38225) at 1/100 dilution + mouse liver tissue lysate

    Secondary
    HRP-anti-rabbit
    Developed using the ECL technique

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38225 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

References for Anti-PKA regulatory subunit I beta antibody (ab38225)

This product has been referenced in:
  • Wong TH  et al. PRKAR1B mutation associated with a new neurodegenerative disorder with unique pathology. Brain 137:1361-73 (2014). Read more (PubMed: 24722252) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"