FunctionThis is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.
Western blot - Anti-PKC alpha antibody  (ab11723)
Predicted band size : 80.6 kDa
Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg) Lanes 2, 6 and 10: PKC alpha knockout HAP1 cell lysate (20 µg) Lanes 3, 7 and 11: K562 cell lysate (20 µg) Lanes 4, 8 and 12: HEK293 cell lysate (20 µg) Lanes 1, 2, 3 and 4: Green signal from target – ab11723 observed at 77 kDa Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa Lanes 9, 10, 11 and 12: Merged (red and green) signal
ab11723 was shown to specifically react with PKC alpha when PKC alpha knockout samples were used. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab11723 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
References for Anti-PKC alpha antibody  (ab11723)
This product has been referenced in:
Cao Y et al. Regulators of G protein signaling RGS7 and RGS11 determine the onset of the light response in ON bipolar neurons. Proc Natl Acad Sci U S A109:7905-10 (2012).
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