Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab4124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Predicted molecular weight: 80 kDa. (AP/HRP). With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.

Target

  • FunctionThis is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Cellular localizationCytoplasm. Cell membrane. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAG6 antibody
    • Aging associated gene 6 antibody
    • aPKC antibody
    • KPCA_HUMAN antibody
    • PKC alpha antibody
    • PKC-A antibody
    • PKC-alpha antibody
    • PKCA antibody
    • PRKACA antibody
    • PRKCA antibody
    • Protein Kinase C alpha antibody
    • Protein kinase C alpha type antibody
    see all

Anti-PKC alpha antibody images



  • Predicted band size : 80 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab4124 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab4124 was shown to recognize PKC alpha when PKC alpha knockout samples were used, along with additional cross-reactive bands. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab4124 and ab8245 (loading control to GAPDH) were diluted to 1/250 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

References for Anti-PKC alpha antibody (ab4124)

This product has been referenced in:
  • Griffon A  et al. Integrative analysis of public ChIP-seq experiments reveals a complex multi-cell regulatory landscape. Nucleic Acids Res 43:e27 (2015). Read more (PubMed: 25477382) »
  • Li L  et al. Hippocampal protein kinase C family members in spatial memory retrieval in the mouse. Behav Brain Res 258C:202-207 (2013). Read more (PubMed: 24075976) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"