Overview

  • Product nameAnti-PKC alpha antibody
    See all PKC alpha primary antibodies
  • Description
    Mouse monoclonal to PKC alpha
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 563-673 of Human PKC alpha

Properties

Applications

Our Abpromise guarantee covers the use of ab57415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 77 kDa.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionThis is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Cellular localizationCytoplasm. Cell membrane. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAG6 antibody
    • Aging associated gene 6 antibody
    • aPKC antibody
    • KPCA_HUMAN antibody
    • PKC alpha antibody
    • PKC-A antibody
    • PKC-alpha antibody
    • PKCA antibody
    • PRKACA antibody
    • PRKCA antibody
    • Protein Kinase C alpha antibody
    • Protein kinase C alpha type antibody
    see all

Anti-PKC alpha antibody images

  • PKC alpha antibody (ab57415) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 77 kDa
    PKC alpha antibody (ab57415) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab57415 at 10 ug/ml staining PKC alpha in human Hella cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-PKC alpha antibody (ab57415)

This product has been referenced in:
  • Baldwin AK  et al. Epithelial-mesenchymal status influences how cells deposit fibrillin microfibrils. J Cell Sci 127:158-71 (2014). WB ; Human . Read more (PubMed: 24190885) »
  • Schober JM  et al. The microtubule-associated protein EB1 maintains cell polarity through activation of protein kinase C. Biochem Biophys Res Commun : (2011). Read more (PubMed: 22120625) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab57415.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"