• Product nameAnti-PKC alpha antibody [M237]
    See all PKC alpha primary antibodies
  • Description
    Mouse monoclonal [M237] to PKC alpha
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC, IHC-FoFr, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant human PKC alpha from amino acids residues within the central region.

  • Positive control
    • Neonatal rat brain tissue and adult mouse brain lysate.


Associated products


Our Abpromise guarantee covers the use of ab86715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 77 kDa).
IP 1/100.
IHC-P 1/500. Antigen retrieval is not essential but may optimise staining.
ICC 1/300.
IHC-FoFr Use at an assay dependent concentration. PubMed: 23374330
ELISA 1/2000.


  • FunctionThis is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Cellular localizationCytoplasm. Cell membrane. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAG6 antibody
    • Aging associated gene 6 antibody
    • aPKC antibody
    • KPCA_HUMAN antibody
    • PKC alpha antibody
    • PKC-A antibody
    • PKC-alpha antibody
    • PKCA antibody
    • PRKACA antibody
    • PRKCA antibody
    • Protein Kinase C alpha antibody
    • Protein kinase C alpha type antibody
    see all

Anti-PKC alpha antibody [M237] images

  • All lanes : Anti-PKC alpha antibody [M237] (ab86715) at 1/1000 dilution

    Lane 1 : Neonatal rat brain lysate
    Lane 2 : Neonatal rat brain lysate, alkaline phosphatase treated

    Predicted band size : 77 kDa
    Observed band size : 82 kDa (why is the actual band size different from the predicted?)
    Additional bands at : <82 kDa (possible IgG).
  • ab86715, at 1/500 dilution, staining PKC alpha in formalin-fixed, paraffin-embedded, citric acid treated adult mouse brain by Immunohistochemistry, secondary stained with anti-mouse HRP followed by DAB detection.

References for Anti-PKC alpha antibody [M237] (ab86715)

This product has been referenced in:
  • Huang JF  et al. Differential neuronal expression of receptor interacting protein 3 in rat retina: involvement in ischemic stress response. BMC Neurosci 14:16 (2013). IHC-FoFr ; Rat . Read more (PubMed: 23374330) »

See 1 Publication for this product

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