Anti-PKC alpha (phospho S657 + Y658) antibody (ab23513)
Key features and details
- Rabbit polyclonal to PKC alpha (phospho S657 + Y658)
- Suitable for: ICC, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-PKC alpha (phospho S657 + Y658) antibody
See all PKC alpha primary antibodies -
Description
Rabbit polyclonal to PKC alpha (phospho S657 + Y658) -
Host species
Rabbit -
Specificity
ab23513 detected PKC alpha and PKC beta I, but not other PKC isoforms in rat brain lysate. -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Human PKC alpha (phospho S657 + Y658).
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General notes
We do not have concentration information for this antibody. It is optimized to work at the dilutions stated on the datasheet.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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WB | (4) |
1/1000. Detects a band of approximately 82 kDa.
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Notes |
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ICC
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 82 kDa. |
Target
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Function
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Cell membrane. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 24680 Rat
- SwissProt: P05696 Rat
- Unigene: 207908 Rat
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Alternative names
- AAG6 antibody
- Aging associated gene 6 antibody
- aPKC antibody
see all
Images
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Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKCα antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKCα (Central region) or anti-phospho-PKCα (Ser-657/Tyr-658).
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Immunocytochemical labeling of PKC phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-PKCα (Ser-657/Tyr-658) (PP1091) antibody in the absence (Left) or presence (Right) of blocking peptide (PX1095). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594
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Immunofluorescence analysis of S2-013 cells, staining PKC alpha (phospho S657 + Y658) with ab23513.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with blocking solution (3% BSA/PBS). Cells were incubated with primary antibody for 1 hour. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab23513 has been referenced in 20 publications.
- Jiang Y et al. Aquaporin 1 mediates early responses to osmotic stimuli in endothelial cells via the calmodulin pathway. FEBS Open Bio 11:75-84 (2021). PubMed: 33125833
- Wu A et al. Delphinidin induces cell cycle arrest and apoptosis in HER-2 positive breast cancer cell lines by regulating the NF-κB and MAPK signaling pathways. Oncol Lett 22:832 (2021). PubMed: 34712357
- Lenkavska L et al. Searching for combination therapy by clustering methods: Stimulation of PKC in Golgi apparatus combined with hypericin induced PDT. Photodiagnosis Photodyn Ther N/A:101813 (2020). PubMed: 32442674
- Zhou L et al. Five Constituents in Psoralea corylifolia L. Attenuate Palmitic Acid-Induced Hepatocyte Injury via Inhibiting the Protein Kinase C-a/Nicotinamide-Adenine Dinucleotide Phosphate Oxidase Pathway. Front Pharmacol 10:1589 (2019). PubMed: 32116659
- Miranda-Silva D et al. Characterization of biventricular alterations in myocardial (reverse) remodelling in aortic banding-induced chronic pressure overload. Sci Rep 9:2956 (2019). PubMed: 30814653