Recombinant Anti-PKC alpha (phospho T497) antibody [EP2608Y] (ab76016)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2608Y] to PKC alpha (phospho T497)
- Suitable for: Dot blot, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PKC alpha (phospho T497) antibody [EP2608Y]
See all PKC alpha primary antibodies -
Description
Rabbit monoclonal [EP2608Y] to PKC alpha (phospho T497) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, NIH/3T3, and C6 whole cell lysates. IHC-P: Human glioma, Mouse kidney, and Rat kidney tissues.
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General notes
This product has switched from a hybridoma to recombinant production method on 2nd August 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2608Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Dot blot |
1/1000.
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WB |
1/100000. Predicted molecular weight: 77 kDa.
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IHC-P |
1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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Dot blot
1/1000. |
WB
1/100000. Predicted molecular weight: 77 kDa. |
IHC-P
1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Cell membrane. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5578 Human
- Entrez Gene: 18750 Mouse
- Entrez Gene: 24680 Rat
- Omim: 176960 Human
- SwissProt: P17252 Human
- SwissProt: P20444 Mouse
- SwissProt: P05696 Rat
- Unigene: 531704 Human
see all -
Alternative names
- AAG6 antibody
- Aging associated gene 6 antibody
- aPKC antibody
see all
Images
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All lanes : Anti-PKC alpha (phospho T497) antibody [EP2608Y] (ab76016) at 1/10000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, then the membrane was incubated with Alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-PKC alpha (phospho T497) antibody [EP2608Y] (ab76016) at 1/10000 dilution
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, then the membrane was incubated with Alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-PKC alpha (phospho T497) antibody [EP2608Y] (ab76016) at 1/10000 dilution
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate, then the membrane was incubated with Alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Exposure time: 5 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Dot blot analysis of PKC alpha (pT497) using ab76016 at 1:1000 (1.34 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:20000 dilution.
Lane 1: PKC alpha non-phospho peptide.
Lane 2: PKC alpha (phospho T497).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
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Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling PKC alpha with ab76016 at 1/10000 (0.134 µg/ml), with or without Lambda phosphatase treatment, then followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human glioma. No signal was detected when tissue was treated with Lambda phosphatase. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 30 mins.
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Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling PKC alpha with ab76016 at 1/10000 (0.134 µg/ml), with or without Lambda phosphatase treatment, then followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Mouse kidney. No signal was detected when tissue was treated with Lambda phosphatase. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 30 mins.
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Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling PKC alpha with ab76016 at 1/10000 (0.134 µg/ml), with or without Lambda phosphatase treatment, then followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Rat kidney. No signal was detected when tissue was treated with Lambda phosphatase. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 30 mins.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab76016 has been referenced in 17 publications.
- Collins HE et al. Cardiomyocyte stromal interaction molecule 1 is a key regulator of Ca2+ -dependent kinase and phosphatase activity in the mouse heart. Physiol Rep 10:e15177 (2022). PubMed: 35179826
- Scerri J et al. PKC-mediated phosphorylation and activation of the MEK/ERK pathway as a mechanism of acquired trastuzumab resistance in HER2-positive breast cancer. Front Endocrinol (Lausanne) 13:1010092 (2022). PubMed: 36329884
- Wang Y et al. A Global Phosphorylation Atlas of Proteins Within Pathological Site of Rotator Cuff Tendinopathy. Front Mol Biosci 8:787008 (2021). PubMed: 35242811
- Kim JE et al. Inhibition of AKT/GSK3ß/CREB Pathway Improves the Responsiveness to AMPA Receptor Antagonists by Regulating GRIA1 Surface Expression in Chronic Epilepsy Rats. Biomedicines 9:N/A (2021). PubMed: 33919872
- Tirronen A et al. The Ablation of VEGFR-1 Signaling Promotes Pressure Overload-Induced Cardiac Dysfunction and Sudden Death. Biomolecules 11:N/A (2021). PubMed: 33802976