Recombinant
RabMAb

Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)

Overview

  • Product name
    Anti-PKC alpha (phospho T638) antibody [E195]
    See all PKC alpha primary antibodies
  • Description
    Rabbit monoclonal [E195] to PKC alpha (phospho T638)
  • Specificity
    The antibody only detects PKC alpha phosphorylated on Threonine 638.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha.

  • Positive control
    • 293 cell lysate and normal brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab32502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Detects a band of approximately 80 kDa (predicted molecular weight: 77 kDa).
IHC-P 1/250.
ICC/IF 1/500.
IP 1/40.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    Images

    • All lanes : Anti-PKC alpha (phospho T638) antibody [E195] (ab32502) at 1/20000 dilution

      Lane 1 : 293 cell lysate, untreated
      Lane 2 : 293 cell lysate, treated with TPA.


      Predicted band size : 77 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of untreated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution.
    • Immunohistochemical staining of Phosphatase-treated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution

    References

    This product has been referenced in:
    • Fan K  et al. Hypomethylation of CNTFRa is associated with proliferation and poor prognosis in lower grade gliomas. Sci Rep 7:7079 (2017). Read more (PubMed: 28765641) »
    • Liu Z  et al. Adiponectin reduces ER stress-induced apoptosis through PPARa transcriptional regulation of ATF2 in mouse adipose. Cell Death Dis 7:e2487 (2016). WB ; Mouse . Read more (PubMed: 27882945) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    We have not yet tested this item for cross reactivity against other PKC family members. BLASTs and immunogen alignment that I have performed show that this product, has 85% and 66% homology against mouse PKC beta and gamma respectively. Alignment again...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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