Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)

Overview

  • Product nameAnti-PKC alpha (phospho T638) antibody [E195]
    See all PKC alpha primary antibodies
  • Description
    Rabbit monoclonal [E195] to PKC alpha (phospho T638)
  • SpecificityThe antibody only detects PKC alpha phosphorylated on Threonine 638.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha.

  • Positive control
    • 293 cell lysate and normal brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Detects a band of approximately 80 kDa (predicted molecular weight: 77 kDa).
IHC-P 1/250.
ICC/IF 1/500.
IP 1/40.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    Anti-PKC alpha (phospho T638) antibody [E195] images

    • All lanes : Anti-PKC alpha (phospho T638) antibody [E195] (ab32502) at 1/20000 dilution

      Lane 1 : 293 cell lysate, untreated
      Lane 2 : 293 cell lysate, treated with TPA.


      Predicted band size : 77 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of untreated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution.
    • Immunohistochemical staining of Phosphatase-treated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution

    References for Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)

    This product has been referenced in:

    See all 5 Publications for this product

    Product Wall

    We have not yet tested this item for cross reactivity against other PKC family members. BLASTs and immunogen alignment that I have performed show that this product, has 85% and 66% homology against mouse PKC beta and gamma respectively. Alignment again...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"