Recombinant
RabMAb

Anti-PKC beta 1 antibody [EPR18512] (ab195039)

Overview

  • Product name
    Anti-PKC beta 1 antibody [EPR18512]
    See all PKC beta 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR18512] to PKC beta 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PKC beta 1 aa 600 to the C-terminus. The exact sequence is proprietary.
    Database link: P05771

  • Positive control
    • WB: Active human PKC beta 1 full length protein; Human fetal brain whole cell lysate; L-929, C2C12, U-87 MG, HeLa, C6 and NIH/3T3 cell lysates; Mouse brain and rat brain lysates. IHC-P: Human colon, Human colon cancer, mouse kidney and rat spleen tissues. ICC/IF: HeLa and K562 cells. Flow Cyt: K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR18512
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab195039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 77, 40 kDa (predicted molecular weight: 77 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.
Flow Cyt 1/200.

Target

  • Function
    Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
  • Cellular localization
    Cytoplasm. Nucleus. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPCB_HUMAN antibody
    • PKC beta antibody
    • PKC-B antibody
    • PKC-beta antibody
    • PKCB antibody
    • Prkcb antibody
    • PRKCB I antibody
    • PRKCB1 antibody
    • PRKCB2 antibody
    • Protein kinase C beta antibody
    • Protein kinase C beta type antibody
    • protein kinase C, beta 1 polypeptide antibody
    • protein kinase C, beta-1 antibody
    see all

Images

  • All lanes : Anti-PKC beta 1 antibody [EPR18512] (ab195039) at 1/2000 dilution

    Lane 1 : Active human PKC alpha full length protein
    Lane 2 : Active human PKC beta 1 full length protein
    Lane 3 : Active human PKC beta 2 full length protein
    Lane 4 : Active human PKC gamma full length protein
    Lane 5 : Active human PKC delta full length protein
    Lane 6 : Active human PKC eta full length protein
    Lane 7 : Active human PKC epsilon full length protein
    Lane 8 : Active human PKC theta full length protein
    Lane 9 : Active human PKC mu full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 77 kDa
    Observed band size: 103 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein (ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag; Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag; Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PKC beta 1 with ab195039 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and weak cytoplasm staining on epithelial cells of Human colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling PKC beta 1 with ab195039 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasm staining on K562 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab195039 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

     

  • Flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling PKC beta 1 with ab195039 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PKC beta 1 with ab195039 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasm staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab195039 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

     

  • All lanes : Anti-PKC beta 1 antibody [EPR18512] (ab195039) at 1/10000 dilution

    Lane 1 : Human fetal brain whole cell lysate
    Lane 2 : L-929 (Mouse connective tissue fibroblast cells) whole cell lysate
    Lane 3 : C2C12 (Mouse myoblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 77 kDa
    Observed band size: 40,77 kDa (why is the actual band size different from the predicted?)


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The peptide immunogen corresponds to the C terminal region of human PKC beta 1. The antibody recognizes the full length (~77KD) and the catalytic domain (~40KD) of PKC beta 1 (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331.

  • All lanes : Anti-PKC beta 1 antibody [EPR18512] (ab195039) at 1/10000 dilution

    Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 77 kDa
    Observed band size: 40,77 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The peptide immunogen corresponds to the C terminal region of human PKC beta 1. The antibody recognizes the full length (~77KD) and the catalytic domain (~40KD) of PKC beta 1 (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331.

  • All lanes : Anti-PKC beta 1 antibody [EPR18512] (ab195039) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 77 kDa
    Observed band size: 40,77 kDa (why is the actual band size different from the predicted?)


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The peptide immunogen corresponds to the C terminal region of human PKC beta 1. The antibody recognizes the full length (~77KD) and the catalytic domain (~40KD) of PKC beta 1 (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331.

  • All lanes : Anti-PKC beta 1 antibody [EPR18512] (ab195039) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 77 kDa
    Observed band size: 40,77 kDa (why is the actual band size different from the predicted?)


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The peptide immunogen corresponds to the C terminal region of human PKC beta 1. The antibody recognizes the full length (~77KD) and the catalytic domain (~40KD) of PKC beta 1 (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling PKC beta 1 with ab195039 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and cytoplasm staining on cancer cells of colon cancer is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling PKC beta 1 with ab195039 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and cytoplasm staining on epithelial cells of mouse kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling PKC beta 1 with ab195039 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and cytoplasm staining on lymphocytes of rat spleen is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

References

ab195039 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab195039.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up