Anti-PKC beta 1 (phospho S661) antibody (ab79307)

Overview

  • Product name
    Anti-PKC beta 1 (phospho S661) antibody
    See all PKC beta 1 primary antibodies
  • Description
    Rabbit polyclonal to PKC beta 1 (phospho S661)
  • Specificity
    Binds PKC beta 1 only when phosphorylated at serine 661
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human PKC beta 1 around the phosphorylation site of serine 661 (GFSPYT)

  • Positive control
    • Extracts from heat shock-treated HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+) , 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by affinity chromatography using an epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 77 kDa.
ELISA 1/20000.

Target

  • Function
    Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
  • Cellular localization
    Cytoplasm. Nucleus. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPCB_HUMAN antibody
    • PKC beta antibody
    • PKC-B antibody
    • PKC-beta antibody
    • PKCB antibody
    • Prkcb antibody
    • PRKCB I antibody
    • PRKCB1 antibody
    • PRKCB2 antibody
    • Protein kinase C beta antibody
    • Protein kinase C beta type antibody
    • protein kinase C, beta 1 polypeptide antibody
    • protein kinase C, beta-1 antibody
    see all

Images



  • Predicted band size : 77 kDa

    Western blot analysis of lysates from HeLa treated with PMA (left), HeLa (middle), and HeLa treated with heat shock (right), using PKC beta 1 (phospho S661) antibody (ab79307).

References

ab79307 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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