Overview

  • Product nameAnti-PKC Consensus antibody
  • Description
    Rabbit polyclonal to PKC Consensus
  • Tested applicationsSuitable for: ELISAmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    Synthetic peptide conjugated to Bovine thyroglobulin, corresponding to amino acids 543-550 of PKC Consensus

Properties

  • FormLyophilised:Reconstitute with 0.1 ml of 10 mg/ml BSA in PBS.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • Concentration information loading...
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notesELISA: Use at an assay dependent dilution.

    With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceSerine/threonine protein kinases phosphorylate the OH group of serine or threonine (which have similar sidechains). Protein kinase C is actually a family of protein kinases consisting of ~10 isozymes. They are divided into three subfamilies: conventional (or classical), novel, and atypical based on their second messenger requirements. While serine/threonine kinases all phosphorylate serine or threonine residues in their substrates, they select specific residues to phosphorylate on the basis of residues that flank the phosphoacceptor site, which together comprise the consensus sequence. Since the consensus sequence residues of the substrate to be phosphorylated make contact with the catalytic cleft of the kinase at several key amino acids (usually through hydrophobic forces and ionic bonds), a kinase is usually not specific to a single substrate, but instead can phosphorylate a whole "substrate family" having common recognition sequences. The consensus sequence of protein kinase C enzymes is similar to that of protein kinase A, since it contains basic amino acids close to the Ser/Thr to be phosphorylated. Their substrates are MARCKS proteins, MAP kinase, transcription factor inhibitor IkappaB, the vitamin D3 receptor VDR, Raf kinase, calpain, and the epidermal growth factor receptor.
    • Cellular localizationCytoplasmic and Plasma membrane
    • Database links
    • Alternative names
      • PKC A antibody
      • PKCA antibody
      • PRKCA antibody

    References for Anti-PKC Consensus antibody (ab40652)

    ab40652 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab40652.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"