Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PKC delta.
(Peptide available as ab97364.)
Our Abpromise guarantee covers the use of ab86800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 77 kDa).|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PKC delta knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab86800 observed at 80 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab86800 was shown to recognize PKC delta when PKC delta knockout samples were used, along with additional cross-reactive bands. Wild-type and PKC delta knockout samples were subjected to SDS-PAGE. ab86800 and ab8245 (loading control to GAPDH) were diluted 1 μg/mL and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"