Overview

  • Product name
    Anti-PKC delta (phospho S645) antibody
    See all PKC delta primary antibodies
  • Description
    Rabbit polyclonal to PKC delta (phospho S645)
  • Specificity
    This antibody detects PKC delta only when phosphorylated at serine 645.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human PKC delta around the phosphorylation site of serine 645 (R-L-SP-Y-S).

  • Positive control
    • IHC-P: Human breast carcinoma tissue. WB: MCF7 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab47773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • Function
    This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Domain
    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
    The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.
  • Post-translational
    modifications
    Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CVID9 antibody
    • D14Ertd420e antibody
    • Kinase PKC delta antibody
    • KPCD antibody
    • KPCD_HUMAN antibody
    • MAY 1 antibody
    • MAY1 antibody
    • MGC49908 antibody
    • nPKC delta antibody
    • nPKC-delta antibody
    • PCKd antibody
    • PKC d antibody
    • PKC delta antibody
    • PKCD antibody
    • PKCdelta antibody
    • PRKC D antibody
    • PRKC delta antibody
    • Prkcd antibody
    • Protein Kinase C delta antibody
    • Protein kinase C delta type antibody
    • Protein kinase C delta VIII antibody
    • Protein Kinase Cdelta antibody
    • Tyrosine protein kinase PRKCD antibody
    see all

Images

  • This image shows human breast carcinoma stained with ab47773 at 1/50 dilution. Right hand image shows tissue treated with the immunogenic phospopeptide, whereas the left hand image is untreated.
  • All lanes : Anti-PKC delta (phospho S645) antibody (ab47773) at 1/500 dilution

    Lane 1 : MCF7 cell extract, untreated
    Lane 2 : MCF7 cell extract treated with immunogenic phosphopeptide


    Predicted band size : 77 kDa

References

This product has been referenced in:
  • Misuth M  et al. The flashlights on a distinct role of protein kinase C d: Phosphorylation of regulatory and catalytic domain upon oxidative stress in glioma cells. Cell Signal 34:11-22 (2017). WB ; Human . Read more (PubMed: 28237688) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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