Anti-PKC delta (phospho Y52) antibody (ab74007)

Overview

  • Product nameAnti-PKC delta (phospho Y52) antibody
    See all PKC delta primary antibodies
  • Description
    Rabbit polyclonal to PKC delta (phospho Y52)
  • SpecificityDetects PKC delta only when phosphorylated at tyrosine 52.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human PKC delta around the phosphorylation site of tyrosine 52 (T-M-YP-P-E)

  • Positive control
    • Human lung carcinoma tissue. Extracts from Jurkat cells. NIH/3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 77 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionThis is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
    The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.
  • Post-translational
    modifications
    Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.
  • Cellular localizationCytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CVID9 antibody
    • D14Ertd420e antibody
    • Kinase PKC delta antibody
    • KPCD antibody
    • KPCD_HUMAN antibody
    • MAY 1 antibody
    • MAY1 antibody
    • MGC49908 antibody
    • nPKC delta antibody
    • nPKC-delta antibody
    • PCKd antibody
    • PKC d antibody
    • PKC delta antibody
    • PKCD antibody
    • PKCdelta antibody
    • PRKC D antibody
    • PRKC delta antibody
    • Prkcd antibody
    • Protein Kinase C delta antibody
    • Protein kinase C delta type antibody
    • Protein kinase C delta VIII antibody
    • Protein Kinase Cdelta antibody
    • Tyrosine protein kinase PRKCD antibody
    see all

Anti-PKC delta (phospho Y52) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab74007, at 1/50 dilution, in the presence (right panel) or absence (left panel) of the immunising phophopeptide.
  • All lanes : Anti-PKC delta (phospho Y52) antibody (ab74007) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells starved for 24hours
    Lane 2 : Extracts from Jurkat cells starved for 24hours with immunising phosphopeptide at 10 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 77 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 115 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of NIH/3T3 cells using ab74007, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising phosphopeptide.

References for Anti-PKC delta (phospho Y52) antibody (ab74007)

ab74007 has not yet been referenced specifically in any publications.

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