Anti-PKC epsilon antibody [17] (ab11724)

Overview

  • Product nameAnti-PKC epsilon antibody [17]
    See all PKC epsilon primary antibodies
  • Description
    Mouse monoclonal [17] to PKC epsilon
  • Specificityab11724 recognises the epsilon isoform of protein kinase C. This antibody recognises the sequence NQEEFKGFSYFGEDLMP at the C terminus of PKC epsilon.
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    C-terminal peptide of human PKC epsilon.

  • General notesThis product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.


    This is a calcium-independent, phospholipid-dependent enzyme specific for serine and threonine. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumour promoters.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tissue Culture Supernatant
  • PurityTissue culture supernatant
  • Primary antibody notesThis is a calcium-independent, phospholipid-dependent enzyme specific for serine and threonine. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumour promoters.
  • ClonalityMonoclonal
  • Clone number17
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/10.
WB 1/10 - 1/500. Predicted molecular weight: 88.4 kDa.

Target

  • FunctionThis is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    modifications
    Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPCE_HUMAN antibody
    • MGC125656 antibody
    • MGC125657 antibody
    • nPKC epsilon antibody
    • nPKC-epsilon antibody
    • PKCE antibody
    • Pkcea antibody
    • PRKCE antibody
    • Protein kinase C epsilon antibody
    • Protein kinase C epsilon type antibody
    see all

References for Anti-PKC epsilon antibody [17] (ab11724)

ab11724 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I am sorry but we do not recommend a positive control lysate for this antibody. However, following some literature searching I have found a publication where a purified form of the protein was used to demonstrate express...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"