Anti-PKC epsilon (phospho S729) antibody (ab63387)


  • Product nameAnti-PKC epsilon (phospho S729) antibody
    See all PKC epsilon primary antibodies
  • Description
    Rabbit polyclonal to PKC epsilon (phospho S729)
  • SpecificityDetects endogenous levels of PKC epsilon only when phosphorylated at serine 729.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human
  • Immunogen

    Synthetic phosphopeptide from around the phosphorylation site of serine 729 (GFSPYF) of human PKC epsilon

  • Positive control
    • HeLa cells treated with PMA (125ng/ml, 30mins) Jurkat cells treated with PMA (125ng/ml, 30mins) NIH/3T3 cells treated with PMA (125ng/ml, 30mins) This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab63387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).
ELISA 1/10000.
IP Use a concentration of 5 µg/ml.


  • FunctionThis is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Post-translational
    Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPCE_HUMAN antibody
    • MGC125656 antibody
    • MGC125657 antibody
    • nPKC epsilon antibody
    • nPKC-epsilon antibody
    • PKCE antibody
    • Pkcea antibody
    • PRKCE antibody
    • Protein kinase C epsilon antibody
    • Protein kinase C epsilon type antibody
    see all

Anti-PKC epsilon (phospho S729) antibody images

  • All lanes : Anti-PKC epsilon (phospho S729) antibody (ab63387) at 1/500 dilution

    Lane 1 : HeLa cells treated with PMA (125ng/ml, 30mins) at 30 µg
    Lane 2 : Jurkat cells treated eith PMA (125ng/ml, 30mins) at 30 µg
    Lane 3 : NIH/3T3 cells treated with PMA (125ng/ml, 30mins) at 30 µg
    Lane 4 : NIH/3T3 cells treated with syntesized peptide (125ng/ml, 30mins) at 10 µg

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
  • PKC epsilon was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to PKC epsilon and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab63387.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 84kDa; PKC epsilon
  • ICC/IF image of ab63387 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab63387 at 5µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PKC epsilon (phospho S729) antibody (ab63387)

This product has been referenced in:
  • Björnström K  et al. Orexin A inhibits propofol-induced neurite retraction by a phospholipase D/protein kinase Ce-dependent mechanism in neurons. PLoS One 9:e97129 (2014). WB ; Rat . Read more (PubMed: 24828410) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (10%)
Sample Guinea pig Cell lysate - other (cardiac myocyte)
Specification cardiac myocyte
Treatment 100nM drug1 for 30min, 1uM drug1 for 30min, 10uM drug1 for 30min, 100uM drug1 for 15min, 100uM drug1 for 30min, 100uM drug1 for 60min
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

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