Anti-PKC eta antibody (ab4134)


  • Product nameAnti-PKC eta antibody
    See all PKC eta primary antibodies
  • Description
    Rabbit polyclonal to PKC eta
  • Tested applicationsSuitable for: ELISA, ICC, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: all Mammals
  • Immunogen

    Synthetic peptide (Rat).



Our Abpromise guarantee covers the use of ab4134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: assay dependent.
    Immunocytochemistry (frozen): 1/250 (using horseradish peroxidase or alkaline phosphatase).
    Western blot: 1/250 (using horseradish peroxidase or alkaline phosphatase). Predicted molecular weight: 82 kDa.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
  • Target

    • FunctionThis is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • Tissue specificityMost abundant in lung, less in heart and skin.
    • Involvement in diseaseDefects in PRKCH may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • KPCL_HUMAN antibody
      • MGC 5363 antibody
      • MGC26269 antibody
      • MGC5363 antibody
      • nPKC eta antibody
      • nPKC-eta antibody
      • PKC h antibody
      • PKC L antibody
      • PKC-L antibody
      • PKCh antibody
      • PKCL antibody
      • Prkch antibody
      • PRKCL antibody
      • Protein kinase C eta antibody
      • Protein kinase C eta type antibody
      see all

    References for Anti-PKC eta antibody (ab4134)

    ab4134 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Blocking step Antibody diluent E09-300 GBI labs as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Sample Mouse Tissue sections (brain)
    Specification brain
    Permeabilization Yes - 0.1% Triton
    Fixative Paraformaldehyde

    Dr. Noa Dai

    Verified customer

    Submitted Jul 24 2013

    I am very happy to hear your customer is interested in submitting an Abreview for testing ab4134 with IHC-Fr in mouse samples.
    In order to raise the discount code I need to know the end user details (name, email and Institution).
    Once you con...

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