Overview

  • Product nameAnti-PKC eta (phospho T655) antibody
    See all PKC eta primary antibodies
  • Description
    Rabbit polyclonal to PKC eta (phospho T655)
  • SpecificityThis antibody does not cross-react with any other PKC isoforms tested.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from a region of human PKC eta that contains threonine 655.

  • Positive control
    • Jurkat cells treated with PMA, a phorbol ester.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: PBS, 1mg/ml BSA. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC eta. The final product is generated by affinity chromatography using a PKC eta-derived peptide that is phosphorylated at threonine 655.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 80 kDa.

Target

  • FunctionThis is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificityMost abundant in lung, less in heart and skin.
  • Involvement in diseaseDefects in PRKCH may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPCL_HUMAN antibody
    • MGC 5363 antibody
    • MGC26269 antibody
    • MGC5363 antibody
    • nPKC eta antibody
    • nPKC-eta antibody
    • PKC h antibody
    • PKC L antibody
    • PKC-L antibody
    • PKCh antibody
    • PKCL antibody
    • Prkch antibody
    • PRKCL antibody
    • Protein kinase C eta antibody
    • Protein kinase C eta type antibody
    see all

Anti-PKC eta (phospho T655) antibody images

  • Peptide Competition and Phosphatase Treatment Lysates prepared from Jurkat cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-9) or treated with lambda phosphatase (10), blocked with a 5% BSA-TBST buffer overnight at 4°C, and incubated with 0.50 µg/mL ab5798 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 10), the non phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), the phosphopeptide immunogen (4), or, the phosphopeptide corresponding to the immunogen from other PKC isoforms (5-9). After washing, membranes were incubated with goat (ab’)2 anti-rabbit IgG HRP-conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKC eta [pT655] blocks the antibody signal. The antibody signal was not blocked by the peptides corresponding to PKC isoforms alpha [pT638], beta 1 [pT642], beta 1 [pT641], gamma [pT655] and zeta [pT560], thereby demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

References for Anti-PKC eta (phospho T655) antibody (ab5798)

This product has been referenced in:
  • Lachmann S  et al. Parvovirus interference with intracellular signalling: mechanism of PKCeta activation in MVM-infected A9 fibroblasts. Cell Microbiol 10:755-69 (2008). Read more (PubMed: 18042254) »
  • Shin OS  et al. Role of novel protein kinase C isoforms in Lyme arthritis. Cell Microbiol 9:1987-96 (2007). WB ; Human . Read more (PubMed: 17394560) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"