Overview

  • Product nameAnti-PKC gamma antibody
    See all PKC gamma primary antibodies
  • Description
    Rabbit polyclonal to PKC gamma
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: all Mammals
  • Immunogen

    Synthetic peptide:

    FEACNYPLELYEVSRYGPSSS

    , corresponding to amino acids 302-322 of Human PKC gamma.

Properties

Applications

Our Abpromise guarantee covers the use of ab4144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
ELISA
IHC-P
IHC-Fr
WB
  • Application notesELISA: assay dependent.
    Immunohistochemistry: 1/250 (using horseradish peroxidase or alkaline phosphatase).
    Western blot (using horseradish peroxidase or alkaline phosphatase): 1/250. Predicted molecular weight: 84 kDa.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.

    It has also been reported to be useful for immunoprecipitation of PKC gamma.
  • Target

    • FunctionThis is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • Tissue specificityExpressed in Purkinje cells of the cerebellar cortex.
    • Involvement in diseaseDefects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • KPCG_HUMAN antibody
      • MGC57564 antibody
      • OTTHUMP00000067291 antibody
      • PKC-gamma antibody
      • PKCC antibody
      • PKCG antibody
      • PRKCG antibody
      • Protein kinase C gamma antibody
      • Protein kinase C gamma polypeptide antibody
      • Protein kinase C gamma type antibody
      • Protein kinase C, gamma antibody
      • SCA 14 antibody
      • SCA14 antibody
      see all

    Anti-PKC gamma antibody images

    References for Anti-PKC gamma antibody (ab4144)

    ab4144 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HT1080 Cell line)
    Specification HT1080 Cell line
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton X-100
    Username

    Abcam user community

    Verified customer

    Submitted Feb 17 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HT1080 Cell line)
    Loading amount 20000 cells
    Specification HT1080 Cell line
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 10 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"