Overview

  • Product nameAnti-PKC gamma antibody
    See all PKC gamma primary antibodies
  • Description
    Rabbit polyclonal to PKC gamma
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human
  • Immunogen

    Synthetic human PKC gamma peptide from within residues 611 - 697.

  • Positive control
    • Hela, A431 cell lysate and mouse, rat brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab71558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 34, 40, 66, 78 kDa (predicted molecular weight: 78 kDa).

Target

  • FunctionThis is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • Tissue specificityExpressed in Purkinje cells of the cerebellar cortex.
  • Involvement in diseaseDefects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPCG_HUMAN antibody
    • MGC57564 antibody
    • OTTHUMP00000067291 antibody
    • PKC-gamma antibody
    • PKCC antibody
    • PKCG antibody
    • PRKCG antibody
    • Protein kinase C gamma antibody
    • Protein kinase C gamma polypeptide antibody
    • Protein kinase C gamma type antibody
    • Protein kinase C, gamma antibody
    • SCA 14 antibody
    • SCA14 antibody
    see all

Anti-PKC gamma antibody images

  • All lanes : Anti-PKC gamma antibody (ab71558) at 1/2000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Rat brain lysate


    Predicted band size : 78 kDa
    Observed band size : 78 kDa
    Additional bands at : 34 kDa,40 kDa,66 kDa. We are unsure as to the identity of these extra bands.
  • ab71558 (1µg/ml) staining PKCg in human Brain: Cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic and membrane staining of neuropil, molecular cells and Purkinje cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab71558 stained Hepp cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71558, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PKC gamma antibody (ab71558)

This product has been referenced in:
  • Liu Y  et al. Mutations in the microtubule-associated protein 1A (Map1a) gene cause Purkinje cell degeneration. J Neurosci 35:4587-98 (2015). IHC ; Mouse . Read more (PubMed: 25788676) »
  • Mukhopadhyay P  et al. MUC4 overexpression augments cell migration and metastasis through EGFR family proteins in triple negative breast cancer cells. PLoS One 8:e54455 (2013). WB ; Human . Read more (PubMed: 23408941) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Guinea pig Tissue lysate - other (Cellebreum)
Specification Cellebreum
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Maren Podszun

Verified customer

Submitted May 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"