Recombinant
RabMAb

Anti-PKC gamma antibody [EPR2750] (ab108961)

Overview

  • Product name
    Anti-PKC gamma antibody [EPR2750]
    See all PKC gamma primary antibodies
  • Description
    Rabbit monoclonal [EPR2750] to PKC gamma
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus in Human PKC gamma.

  • Positive control
    • Human fetal brain and Human fetal cerebellum cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 78 kDa).
ICC 1/50.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • Tissue specificity
      Expressed in Purkinje cells of the cerebellar cortex.
    • Involvement in disease
      Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
    • Sequence similarities
      Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • KPCG_HUMAN antibody
      • MGC57564 antibody
      • OTTHUMP00000067291 antibody
      • PKC-gamma antibody
      • PKCC antibody
      • PKCG antibody
      • PRKCG antibody
      • Protein kinase C gamma antibody
      • Protein kinase C gamma polypeptide antibody
      • Protein kinase C gamma type antibody
      • Protein kinase C, gamma antibody
      • SCA 14 antibody
      • SCA14 antibody
      see all

    Images

    • All lanes : Anti-PKC gamma antibody [EPR2750] (ab108961) at 1/1000 dilution

      Lane 1 : Human fetal brain cell lysate
      Lane 2 : Human fetal cerebellum cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 78 kDa

    References

    ab108961 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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