Anti-PKC gamma (phospho T514) antibody [EP2730Y] (ab109539)


  • Product nameAnti-PKC gamma (phospho T514) antibody [EP2730Y]
    See all PKC gamma primary antibodies
  • Description
    Rabbit monoclonal [EP2730Y] to PKC gamma (phospho T514)
  • Tested applicationsSuitable for: Dot blot, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding threonine 514 of Human PKC gamma.

  • Positive control
    • HeLa cell lysate; Human brain tissue
  • General notes

    A trial size is available to purchase for this antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 78 kDa.
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or ICC/IF.
  • Target

    • FunctionThis is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • Tissue specificityExpressed in Purkinje cells of the cerebellar cortex.
    • Involvement in diseaseDefects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA).
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • KPCG_HUMAN antibody
      • MGC57564 antibody
      • OTTHUMP00000067291 antibody
      • PKC-gamma antibody
      • PKCC antibody
      • PKCG antibody
      • PRKCG antibody
      • Protein kinase C gamma antibody
      • Protein kinase C gamma polypeptide antibody
      • Protein kinase C gamma type antibody
      • Protein kinase C, gamma antibody
      • SCA 14 antibody
      • SCA14 antibody
      see all

    Anti-PKC gamma (phospho T514) antibody [EP2730Y] images

    • All lanes : Anti-PKC gamma (phospho T514) antibody [EP2730Y] (ab109539) at 1/5000 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate whole cell lysates
      Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate whole cell lysates. The membrane was then incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 78 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of PKC gamma (pT514) phospho peptide (Lane 1) and PKC gamma non-phospho peptide (Lane 2) using ab109539 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

      Blocking and Diluting buffer and concentration: 5% NFDM /TBST.

      Exposure time: 3 minutes.

    • All lanes : Anti-PKC gamma (phospho T514) antibody [EP2730Y] (ab109539) at 0.02 µg/ml

      Lane 1 : HeLa cell lysate with PKC gamma (pT514) peptide
      Lane 2 : HeLa cell lysate with PKC gamma non-phospho peptide
      Lane 3 : HeLa cell lysate

      Predicted band size : 78 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

      Exposure time : 15 seconds
    • ab109539 at 1/100 dilution staining PKC gamma (phospho T514) in paraffin-embedded Human brain tissue by Immunohistochemistry.

    References for Anti-PKC gamma (phospho T514) antibody [EP2730Y] (ab109539)

    This product has been referenced in:
    • Huang L  et al. Ketamine potentiates hippocampal neurodegeneration and persistent learning and memory impairment through the PKC?-ERK signaling pathway in the developing brain. Brain Res 1476:164-71 (2012). WB . Read more (PubMed: 22985497) »
    • Dunn EF & Connor JH Dominant inhibition of Akt/protein kinase B signaling by the matrix protein of a negative-strand RNA virus. J Virol 85:422-31 (2011). Read more (PubMed: 20980511) »

    See all 2 Publications for this product

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