Overview

  • Product nameAnti-PKC iota antibody
    See all PKC iota primary antibodies
  • Description
    Mouse monoclonal to PKC iota
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-588 of Human PKC iota

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCalcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. May play a role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. Functions in both pro- and anti-apoptotic pathways. Functions in the RAC1/ERK signaling required for transformed growth. Plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs).
    • Tissue specificityPredominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 OPR domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • DomainThe OPR domain mediates interaction with SQSTM1.
      The C1 domain does not bind diacylglycerol (DAG).
    • Post-translational
      modifications
      On neuronal growth factor (NGF) stimulation, phosphorylated by Src on Tyr-265, Tyr-280 and Tyr-334. Phosphorylation on Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation.
    • Cellular localizationCytoplasm. Membrane. Endosome. Nucleus. Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesicular tubular clusters. Transported to VTCs through interaction with RAB2A.
    • Information by UniProt
    • Database links
    • Alternative names
      • aPKC lambda/iota antibody
      • aPKC-lambda/iota antibody
      • Atypical protein kinase C lambda/iota antibody
      • Atypical protein kinase C-lambda/iota antibody
      • DXS1179E antibody
      • KPCI_HUMAN antibody
      • MGC26534 antibody
      • nPKC iota antibody
      • nPKC-iota antibody
      • nPKCiota antibody
      • PKC lambda antibody
      • PKC lambda/iota antibody
      • PKCI antibody
      • PKCiota antibody
      • PKClambda antibody
      • PRKC iota antibody
      • PRKC lambda/iota antibody
      • PRKC-lambda/iota antibody
      • PRKCI antibody
      • Protein kinase C iota antibody
      • Protein kinase C iota type antibody
      see all

    Anti-PKC iota antibody images

    • Western blot against tagged recombinant protein immunogen using ab57422 PKC iota antibody at 1ug/ml. Predicted band size of immunogen is 91 kDa

    References for Anti-PKC iota antibody (ab57422)

    ab57422 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab57422.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"