Overview

  • Product nameAnti-PKC iota antibody
    See all PKC iota primary antibodies
  • Description
    Mouse monoclonal to PKC iota
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MSHTVAGGGS GDHSHQVRVK AYYRGDIMIT HFEPSISFEG LCNEVRDMCS FDNEQLFTMK WIDEEGDPCT VSSQLELEEA FRLYELNKDS ELLIHVFPCV , corresponding to amino acids 1-101 of Human PKC iota

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCalcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. May play a role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. Functions in both pro- and anti-apoptotic pathways. Functions in the RAC1/ERK signaling required for transformed growth. Plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs).
    • Tissue specificityPredominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 OPR domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
    • DomainThe OPR domain mediates interaction with SQSTM1.
      The C1 domain does not bind diacylglycerol (DAG).
    • Post-translational
      modifications
      On neuronal growth factor (NGF) stimulation, phosphorylated by Src on Tyr-265, Tyr-280 and Tyr-334. Phosphorylation on Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation.
    • Cellular localizationCytoplasm. Membrane. Endosome. Nucleus. Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by cSrc, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Vesicular tubular clusters. Transported to VTCs through interaction with RAB2A.
    • Information by UniProt
    • Database links
    • Alternative names
      • aPKC lambda/iota antibody
      • aPKC-lambda/iota antibody
      • Atypical protein kinase C lambda/iota antibody
      • Atypical protein kinase C-lambda/iota antibody
      • DXS1179E antibody
      • KPCI_HUMAN antibody
      • MGC26534 antibody
      • nPKC iota antibody
      • nPKC-iota antibody
      • nPKCiota antibody
      • PKC lambda antibody
      • PKC lambda/iota antibody
      • PKCI antibody
      • PKCiota antibody
      • PKClambda antibody
      • PRKC iota antibody
      • PRKC lambda/iota antibody
      • PRKC-lambda/iota antibody
      • PRKCI antibody
      • Protein kinase C iota antibody
      • Protein kinase C iota type antibody
      see all

    Anti-PKC iota antibody images



    • Predicted band size : 67 kDa
      PKC iota antibody (ab57423) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    References for Anti-PKC iota antibody (ab57423)

    This product has been referenced in:
    • Mashukova A  et al. Rescue of atypical protein kinase C in epithelia by the cytoskeleton and Hsp70 family chaperones. J Cell Sci 122:2491-503 (2009). WB . Read more (PubMed: 19549684) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"