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Product name Anti-PKC mu antibody [EP1493Y] See all PKC mu primary antibodies
Rabbit monoclonal [EP1493Y] to PKC mu
Tested applications Suitable for:
WB more details Unsuitable for:
Flow Cyt or IP
Mouse, Rat, Human
A synthetic peptide corresponding to residues within human PKC mu was used as an immunogen.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EP1493Y
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes WB: 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 102 kDa).
Is unsuitable for Flow Cyt, IHC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
Sequence similarities Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.
Cellular localization Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
Information by UniProt
nPKC D1 antibody
nPKC mu antibody
Anti-PKC mu antibody [EP1493Y] images
Western blot - Anti-PKC mu antibody [EP1493Y] (ab51246)
All lanes : Anti-PKC mu antibody [EP1493Y] (ab51246) at 1/1000 dilution Lane 1 : HeLa cell lysate, untreated Lane 2 : HeLa cell lysate, treated with PMA Lysates/proteins at 10 µg per lane. Secondary Goat anti-rabbit HRP labeled at 1/2000 dilution Predicted band size : 102 kDa Observed band size : 105 kDa ( why is the actual band size different from the predicted?)
References for Anti-PKC mu antibody [EP1493Y] (ab51246)
This product has been referenced in:
et al. Protein kinase D1 mediates stimulation of DNA synthesis and proliferation in intestinal epithelial IEC-18 cells and in mouse intestinal crypts. J Biol Chem 286:511-20 (2011).
Read more (PubMed: 21051537) »
See 1 Publication
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"