Anti-PKC mu/PKD (phospho S738) antibody (ab131101)
Key features and details
- Rabbit polyclonal to PKC mu/PKD (phospho S738)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PKC mu/PKD (phospho S738) antibody
See all PKC mu/PKD primary antibodies -
Description
Rabbit polyclonal to PKC mu/PKD (phospho S738) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human PKC mu/PKD (phospho S738) conjugated to keyhole limpet haemocyanin.
Database link: Q15139 -
Positive control
- MCF cell extract untreated and treated with PMA; HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab131101 was purified by affinity-chromatography using epitope-specific phosphopetide. Non-phospho specific antibodies were removed by chromatography using non-phosphopetide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 102 kDa).
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 102 kDa). |
ICC/IF
1/100 - 1/200. |
Target
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Function
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation. - Information by UniProt
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Database links
- Entrez Gene: 5587 Human
- Omim: 605435 Human
- SwissProt: Q15139 Human
- Unigene: 508999 Human
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Alternative names
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
see all
Images
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All lanes : Anti-PKC mu/PKD (phospho S738) antibody (ab131101) at 1/500 dilution
Lane 1 : MCF cell extract untreated
Lane 2 : MCF cell extract treated with PMA
Predicted band size: 102 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of methanol-fixed HeLa cells labelling PKC mu/PKD (phospho S738) with ab131101 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab131101 has been referenced in 1 publication.
- Li J et al. Upregulated microRNA-31 inhibits oxidative stress-induced neuronal injury through the JAK/STAT3 pathway by binding to PKD1 in mice with ischemic stroke. J Cell Physiol 235:2414-2428 (2020). PubMed: 31517390