Anti-PKC mu (phospho S738 + S742) antibody (ab74105)

Overview

  • Product name
    Anti-PKC mu (phospho S738 + S742) antibody
    See all PKC mu primary antibodies
  • Description
    Rabbit polyclonal to PKC mu (phospho S738 + S742)
  • Specificity
    ab74105 detects endogenous levels of PKC mu only when phosphorylated at serine 738 and serine 742.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthesized phosphopeptide derived from human PKC mu around the phosphorylation site of serine 738 and serine 742 (E-K-SP-F-R-R-SP-V-V).

  • Positive control
    • WB: extracts from A549 cells treated with PMA (125ng/ml, 30mins), and HT-29 cells treated with serum (20%, 15mins). IHC-P: human heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab74105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 102 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • KPCD1_HUMAN antibody
    • nPKC D1 antibody
    • nPKC mu antibody
    • nPKC-D1 antibody
    • nPKC-mu antibody
    • nPKCD1 antibody
    • nPKCmu antibody
    • PKC antibody
    • PKC MU antibody
    • PKCM antibody
    • PKCmu antibody
    • PKD 1 antibody
    • PKD antibody
    • PKD1 antibody
    • PRKCM antibody
    • PRKD 1 antibody
    • Prkd1 antibody
    • Protein kinase C mu antibody
    • Protein kinase C mu type antibody
    • Protein kinase D antibody
    • Protein kinase D1 antibody
    • Serine/threonine protein kinase D1 antibody
    • Serine/threonine-protein kinase D1 antibody
    see all

Images

  • All lanes : Anti-PKC mu (phospho S738 + S742) antibody (ab74105) at 1/500 dilution

    Lane 1 : Extracts from A549 cells treated with PMA (125ng/ml, 30mins), plus phosphopeptide at 10 µg
    Lane 2 : Extracts from HT-29 cells treated with serum (20%, 15mins), plus phosphopeptide at 10 µg
    Lane 3 : Extracts from A549 cells treated with PMA (125ng/ml, 30mins), plus serum (20%, 15mins) and phopeptide at 10 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 102 kDa
    Observed band size : 102 kDa
  • ab74105, at a 1/50 dilution, staining PKC mu in paraffin embedded human heart tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.

References

This product has been referenced in:
  • Pearen MA  et al. The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue. Mol Endocrinol 26:372-84 (2012). WB ; Mouse . Read more (PubMed: 22282471) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Rat Cell lysate - whole cell (Astrocytes)
Specification
Astrocytes
Treatment
10 nM ofControl, Insulin, IGF-1 or both hormones for 15 minutes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Edwin Hernandez

Verified customer

Submitted May 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up