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Rabbit polyclonal to PKC mu (phospho S738 + S742)
ab74105 detects endogenous levels of PKC mu only when phosphorylated at serine 738 and serine 742.
Predicted to work with:
Synthesized phosphopeptide derived from human PKC mu around the phosphorylation site of serine 738 and serine 742 (E-K-S
WB: extracts from A549 cells treated with PMA (125ng/ml, 30mins), and HT-29 cells treated with serum (20%, 15mins).
IHC-P: human heart tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ or Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab74105 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against
non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 102 kDa.
1/50 - 1/100.
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
Information by UniProt
nPKC D1 antibody
nPKC mu antibody
Western blot - PKC mu (phospho S738 + S742) antibody (ab74105)
All lanes : Anti-PKC mu (phospho S738 + S742) antibody (ab74105) at 1/500 dilution Lane 1 : Extracts from A549 cells treated
with PMA (125ng/ml, 30mins), plus phosphopeptide at 10 µg Lane 2 : Extracts from HT-29 cells treated
with serum (20%, 15mins), plus phosphopeptide at 10 µg Lane 3 : Extracts from A549 cells treated
with PMA (125ng/ml, 30mins), plus serum (20%, 15mins) and phopeptide at 10 µg Lysates/proteins at 5 µg per lane. Predicted band size : 102 kDa Observed band size : 102 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKC mu (phospho S738 + S742) antibody (ab74105)
ab74105, at a 1/50 dilution, staining PKC mu in paraffin embedded human heart tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.
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