Recombinant
RabMAb

Anti-PKC mu (phospho S738 + S742) antibody [EP1493-62] (ab75878)

Overview

  • Product name
    Anti-PKC mu (phospho S738 + S742) antibody [EP1493-62]
    See all PKC mu primary antibodies
  • Description
    Rabbit monoclonal [EP1493-62] to PKC mu (phospho S738 + S742)
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding serine 738 and 742 of human PKC mu.

  • Positive control
    • HeLa cell lysate treated with TPA.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab75878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 102 kDa.
IP 1/30.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function
      Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
      Contains 1 PH domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • Cellular localization
      Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
    • Information by UniProt
    • Database links
    • Alternative names
      • KPCD1_HUMAN antibody
      • nPKC D1 antibody
      • nPKC mu antibody
      • nPKC-D1 antibody
      • nPKC-mu antibody
      • nPKCD1 antibody
      • nPKCmu antibody
      • PKC antibody
      • PKC MU antibody
      • PKCM antibody
      • PKCmu antibody
      • PKD 1 antibody
      • PKD antibody
      • PKD1 antibody
      • PRKCM antibody
      • PRKD 1 antibody
      • Prkd1 antibody
      • Protein kinase C mu antibody
      • Protein kinase C mu type antibody
      • Protein kinase D antibody
      • Protein kinase D1 antibody
      • Serine/threonine protein kinase D1 antibody
      • Serine/threonine-protein kinase D1 antibody
      see all

    Images

    • All lanes : Anti-PKC mu (phospho S738 + S742) antibody [EP1493-62] (ab75878) at 1/500 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with TPA

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)

    References

    ab75878 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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