Overview

  • Product nameAnti-PKC theta antibody
    See all PKC theta primary antibodies
  • Description
    Rabbit polyclonal to PKC theta
  • Tested applicationsSuitable for: ELISA, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: all Mammals
  • Immunogen

    Synthetic peptide corresponding to a distinct C terminal region (Rat). H-S-N-F-S-F-I-N-P-G-M-E-T-L-I-C-S

Properties

Applications

Our Abpromise guarantee covers the use of ab4133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-P
IHC-Fr
WB
  • Application notesELISA: assay dependent.
    Immunohistochemistry: 1/250 (alkaline phosphatase or horseradish peroxidase).
    Western blot (enhanced chemiluminescence: 1/250 (alkaline phosphatase or horseradish peroxidase). Predicted molecular weight: 85 kDa.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    With a more sensitive detection system, such as
    enhanced chemiluminescence or ELISA, the antibody may be diluted further.

    It has also been reported to be useful for
    immunoprecipitation of PKC theta.
  • Target

    • FunctionThis is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
      PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
    • Tissue specificitySkeletal muscle, megakaryoblastic cells and platelets.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • DomainThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
    • Post-translational
      modifications
      Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
    • Information by UniProt
    • Database links
    • Alternative names
      • KPCT_HUMAN antibody
      • MGC126514 antibody
      • MGC141919 antibody
      • nPKC theta antibody
      • nPKC-theta antibody
      • nPKCtheta antibody
      • OTTHUMP00000043364 antibody
      • OTTHUMP00000043365 antibody
      • PKC 0 antibody
      • PKC0 antibody
      • Prkcq antibody
      • PRKCT antibody
      • Protein kinase C theta antibody
      • Protein kinase C theta type antibody
      • Protein Kinase Ctheta antibody
      see all

    References for Anti-PKC theta antibody (ab4133)

    ab4133 has not yet been referenced specifically in any publications.

    Product Wall



    The amino acid sequence is H-S-N-F-S-F-I-N-P-G-M-E-T-L-I-C-S

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"